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A Kaposi’s sarcoma-associated herpesvirus/human herpesvirus 8 ORF50 deletion mutant is defective for reactivation of latent

Naive TIME cell cultures were inoculated with equal volumes of PEL culture media, and immunofluorescence staining was performed to detect latency-associated nuclear antigen (LANA) 2 days postinfection (see Materials and Methods). Colocalization of vIRF-1 with the mitochondria was significantly enhanced by VSV infection, with little or no colocalization with peroxisome or MAM markers. Reed, J. VKORC1 (variant 1) is a 159-amino-acid protein that catalyzes the reduction of vitamin K epoxide in the vitamin K cycle, which is involved in carboxylation of several proteins, including blood coagulation factors (18). Naïve TIME cultures were inoculated with ultracentrifuge-concentrated virus from Dox-treated HHV-8+ TIME-TRE/RTA cell culture media, cumulatively collected over 5 days following Dox addition. A competition assay was used to confirm the sequence specific binding of Rta to the PAN promoter. Bull Soc Pathol Exot 94: 231–234.

Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi’s sarcoma. Colocalization of RFP and GFP signals was evident in all but calnexin depleted cells overexpressing gp130. Cells were processed and FAK phosphorylation was measured as described for panel A. Reduction in anti-ORF-65 antibody correlated with clinical improvements, but LNA showed a variable pattern. Lane 1 represents 10% of the input volume of in vitro-translated PKR (p68) used for the binding reactions. Dose response of ORF74-induced activation of NF-κB in KSIMM cells. 5A; an example of IFA with a blood donor specimen is shown in Fig.

1, anti-gB sera could immunoprecipitate an approximately 120-kDa protein from CHO cells expressing HHV-8 gB (lane 2) but not from cells transfected with a vector (lane 1). Axillary lymph node with overt human herpesvirus 8 (HHV8)–positive plasmablastic lymphoma (case 14.2). Kreuter et al. 3B, right side, lanes 2 and 3). (a) DNA PCR amplification of the HHV8-specific KS330233 sequence from serial dilutions of HHV8-exposed DMVEC (+HHV8). GST pull-down assays.One milliliter of crude Escherichia coli lysate was incubated with 30 μl of preswollen glutathione-Sepharose beads (1:1 [wt/vol] in 1× NETN+ [20 mM Tris {pH 7.5}, 100 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40]) supplemented with protease inhibitors (Sigma) at 4°C for 2 h. Moreover, low concentrations of IPC were positive for all specimens tested, further ruling out PCR inhibition.

Protein A-Sepharose-precipitated material was analyzed by Western blot for the detection of vIL-6. ↵*Corresponding author. Approximately 2.5 million cells were transfected with 5 μg of NF-κB–luciferase reporter construct and with 10 μg of pSG5-K1 or pSG5 alone by electroporation (Gene Pulser II; Bio-Rad Laboratories) at 280 V and 960 μF in 250 μL of complete medium, resuspended in 6 mL of medium, and incubated for 48 hours. Positivity, green fluorescence restricted to approximately 30% of the cells is seen in the positive control and the AIDS patient only. Patrick S. in this issue of the Journal provides evidence that valganciclovir can also inhibit replication of human herpesvirus-8 (HHV-8), another y herpesvirus [6]. Similar findings have been reported by other authors; however, the role of genetic factors in KS development and/or HHV-8 infection are far from being established [6, 7].

Thus, the genes for LANA (open reading frame 73 [ORF73] product), kaposin (ORF K12 product), cyclin D (ORF72 product), vFLIP (ORF K13 product), and vIL-6 (ORF K2 product), in addition to some other genes, are expressed, to various degrees, in PEL cell lines or effusions that have been investigated (12, 27, 29,32, 35, 37). This fusion is triggered by an acidic-pH-dependent conformational change of viral glycoprotein(s) and allows release of capsid into the cytoplasm. None of these investigators could confirm this association, but one group ( 4 ) did detect HHV-8 in 18 of 20 acetone-fixed and paraffin-embedded bone marrow biopsy samples tested. Kaposi’s sarcoma (KS) is the most common cancer in HIV-1-infected persons and is caused by one of only 7 human cancer viruses, i.e., human herpesvirus 8 (HHV-8). We excluded persons taking antivirals during sampling or any prior use of antiretrovirals in those who were HIV-infected. This critical literature review explores the pathogenic potential of these genes within the framework of current knowledge of the basic herpesvirology of KSHV, including the relationships between viral genotypic variation and the four clinicoepidemiologic forms of Kaposi’s sarcoma, current viral detection methods and their utility, primary infection by KSHV, tissue culture and animal models of latent- and lytic-cycle gene expression and pathogenesis, and viral reactivation from latency. ^ Moore PS, Gao SJ, Dominguez G, Cesarman E, Lungu O, Knowles DM, Garber R, Pellett PE, McGeoch DJ, Chang Y (January 1996).

We also found that the level of antibodies directed against lytic or latent HHV-8 antigens is not correlated to the amount of HHV-8 viral load in the PBMCs, and finally, we demonstrated a much higher viral load in tumoral skin lesions (6.07 log copies/μg DNA) than in unaffected skin (2.93 log copies/μg DNA) or in PBMCs (2.55 log copies/μg DNA). To achieve this goal, PBMC and purified cell populations from 45 patients with KS and 45 patients at risk of KS were analyzed for HHV-8 DNA and/or gene expression and for cell survival, growth, and phenotype before or after culture with or without the IC increased in KS.