Quantitative Analysis of Human Herpesvirus 8 Viral Load Using a Real-Time PCR Assay

227-SR). The region comprising amino acids 40 to 48 of VKORC1v2 (PΔ5-Δ6) was able to interact with CatD as effectively as the Δ5 construction, consisting of residues 40 to 76, used as a positive control. Results are presented as the relative firefly luciferase activity over the Renilla luciferase activity. Elevated HIV RNA levels may play a role in inducing HHV-8 reactivation through either increased immunosuppression or direct activation of HHV-8 itself. In addition, multiple cell types associated with the dermal and epidermal layers in KS lesions support their role in productive HHV-8 infection. PCR was performed on serum samples of patient 2, evaluating the HHV‐8 minor capsid (ORF 26), from nucleotides 47311 to 47384 of the HHV‐8 genome, as described previously by White and Campbell 18. The right panels present the results of an analogous experiment, showing specificity of VKORC1v2 binding to vIL-6; no interaction between VKORC1v2 and ER protein calreticulin (Crt) was detected.

2 ). 4A). The 83 sera of the training set, 43 controls (CTRL) and 40 KS/HHV-8-infected subjects (KS), were reblinded and retested with a new five-antigen mixture employing K8.1-Δ4 instead of K8.1-Δ1 and K8.1-Δ2 (A), as well as with K8.1-Δ4 alone (B). Three serum samples found to be negative by ELISA using the ORF73 protein as the antigen reacted with the K8.1 or ORF65 protein. PCR-based in situ lysis gel analysis of HHV-8 genomes during initial infection. This level was sustained until at least 48 h postinduction. Moreover, infected histocultures released infectious virus particles (data not shown) suggesting that placental cells can support the entire lytic replication cycle.

Since MIP-2 is regulated in part by NF-κB (45), we asked whether Tat increased the level of MIP-2 mRNA expression in GR3 cells. One of the animals (animal 116) with a tail tumor was mated to produce an F2 generation. However, the heterogeneous, multiclonal cellular composition of KS, the involution of KS following an immune response, the probably important role of infiltrating inflammatory cells, and the key role that the release of inflammatory and angiogenic mediators by KSHV-infected cells has in driving KS tumorigenesis (see below), exemplify an inflammatory-driven oncogenic process or paracrine neoplasia62. Multiple concomitant viral infections might also enhance the lytic-cycle spread of emerging infectious pathogens, such as HHV-8. Figure 1. After extensive washing, endogenous IKK activity was determined using GST-IκBα (1-54) as a substrate, as described.33 Western blot analysis for IKK was performed as kinase loading control. The HIV-1 transactivator protein (Tat) is also hypothesized to contribute to this interplay, although additional, as yet unknown factors may also be required to complete the HHV-8 lytic transformation and progression to KS [45, 46].

Significant responses were seen in patients treated with the matrix-metalloprotein inhibitor COL-3 and in 2 trials of imatinib. Postnuclear supernatants were applied and centrifugation carried out at 25,000 rpm in an SW41 Ti rotor for 3 h at 4°C. Here we present several lines of evidence to show that gB, independently of other viral glycoproteins, induces the FAK-Src-PI-3K-Rho GTPase signaling pathway and extensive cytoskeletal rearrangement in target cells. HHV-8 lytic infection in vitro and induction of a cytokine-chemokine response in B cells. HHV-8 seroprevalence was unaffected by pretransplant therapeutic regimens, a finding supported by other studies showing a low rate of parenteral HHV-8 transmission (7, 12, 16). Patient 1 was HHV-8 seronegative before transplantation and received kidney transplantation in another country (donor serum not available for analysis). (A) HHV-8 titers following gp130 depletion in TRExBCBL1-RTA cells.

According to Nakamura et al,5 CD4-positive lymphocytes, infected with HIV or HTLV-1, secrete a potential mitogen which stimulates the growth of KS. 340:1045-1046, 1999). Studies have mainly concerned patients with AIDS. The conditioning regimen consisted of BEAM chemotherapy (carmustine, etoposide, aracytine, and melphalan). HHV-8 homologues were also detected in drill, mandrill, and a hybrid of Mandrillus leucophaeus-Mandrill sphinx, nonhuman primates living in Cameroon and Gabon, central Africaref. The 1.2-kb hIL-6 promoter region was amplified from total cellular DNA using primers F (5′-GGAAGATCTCTCCTGCAAGAGACACCATCCTGA-3′) and R (5′-CGGGAATTCAGGGCAGAATGAGCCTCAGAGACAT3-3′); the underlined nucleotides represent BglII and EcoRI sites, respectively. Additionally, quantity of HHV-8 detected tended to be more homogeneous compared to HSV-2, with the vast majority of swabs clustered around 4.5 log copies/mL, whereas HSV-2 is frequently detected at both lower and much higher quantities [18, 19].

We found that the expression of vIRF-2 in HEK293 cells inhibited the antiviral effect of interferon and rescued translation of vesicular stomatitis virus mRNA from interferon-induced translational block. Multicentric Castleman disease (CD), primary effusion lymphoma, and Kaposi sarcoma are 3 diseases associated with human herpesvirus (HHV)-8 infection that occur in HIV-infected and HIV-uninfected patients. This method allowed us to quantify precisely the average HHV-8 copy number per cell in various persistently HHV-8-infected cell lines (BBG-1 cells, n= 200; BC-1 cells, n = 59; BCBL-1 cells,n = 70). Epidemiologic evidence and the recent identification of herpesvirus-like DNA sequences in patients with KS have suggested a role for viral agents in the etiopathogenesis of this disease.